skov3 human epithelial ovary cancer cell line  (ATCC)

Journal: BMC Women's Health

Article Title: The inhibitory effect of curcumin via fascin suppression through JAK/STAT3 pathway on metastasis and recurrence of ovary cancer cells

doi: 10.1186/s12905-020-01122-2

Figure Lengend Snippet: Attachment assay of SKOV3 cells treated with 10 and 20 μM curcumin for 6 h. Once the curcumin was washed out, the attachment assays were performed after 6, 24, and 48 h

Article Snippet: Cell lines were derived from the SKOV3 human epithelial ovary cancer cell line (ATCC, Manassas, VA, United States) [ ].

Techniques:

Journal: Journal of cancer therapy

Article Title: Oncomorphic TP 53 Mutations in Gynecologic Cancers Lose the Normal Protein:Protein Interactions with the microRNA Microprocessing Complex

doi: 10.4236/jct.2014.56058

Figure Lengend Snippet: Mutant p53 proteins lose the interaction with the microprocessing Drosha complex. (a) Co-IP experiments demonstrated a loss of interaction with the Drosha complex in cell models expressing various mutated forms of p53. SKOV3 cell lines (p53 null) stably expressed p53 mutant proteins (6 oncomorphic mutations (R175H, R248Q, R248Q.P72R, R273C, R273L, and R273S) and 1 unclassified mutation (I195T). IP of DDX5 or p53 detected binding of p53 in the WT UCI-107 cell lines, but not in any other cell lines with mutant p53 proteins. (b) WT p53-responsive miRNA expression in SKOV3 cells expressing R248Q.P72R p53 mutant at a time course after radiation treatment.

Article Snippet: The human ovary cancer cell line SKOV3 was purchased from American Tissue Culture Collection (ATCC, Bethesda, MD).

Techniques: Mutagenesis, Co-Immunoprecipitation Assay, Expressing, Stable Transfection, Binding Assay